Project

Part:BBa_J5530:Design

Designed by: Charles Yoon   Group: iGEM06_Toronto   (2006-10-30)


Cell-See-Us Bacterial Thermometer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2383
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 5026
    Illegal AgeI site found at 5138
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4169
    Illegal SapI site found at 961


Design Notes

Addition of external control factors such as IPTG and aTc to modulate GFP and mRFP signal strengths.


Source

iGEM 2006 Registry

References